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SMA20/PMIS2 is unique to Eutheria. Shown are dotplot comparisons constructed with a concatemeric assembly of armadillo reverse-complemented Atp4a , Pmis2 , and Haus5 coding sequences (cds) as the vertical dimension for interrogation of armadillo and opossum genomic loci, and a comparable assembly with reverse-complemented Atp4a , Pmis2 , and reverse-complemented Etv2 cds for interrogation of platypus genomic loci.

Journal: International Journal of Molecular Sciences

Article Title: SMA20/PMIS2 Is a Rapidly Evolving Sperm Membrane Alloantigen with Possible Species-Divergent Function in Fertilization

doi: 10.3390/ijms25073652

Figure Lengend Snippet: SMA20/PMIS2 is unique to Eutheria. Shown are dotplot comparisons constructed with a concatemeric assembly of armadillo reverse-complemented Atp4a , Pmis2 , and Haus5 coding sequences (cds) as the vertical dimension for interrogation of armadillo and opossum genomic loci, and a comparable assembly with reverse-complemented Atp4a , Pmis2 , and reverse-complemented Etv2 cds for interrogation of platypus genomic loci.

Article Snippet: We also queried the NCBI Gene database (10 November 2024) to retrieve the pig and other species’ syntenic regions spanning Atp4a – Haus5 and conducted pairwise dotplot comparisons (MegAlign program of the Lasergene 15 software suite; DNAStar, Madison, WI, USA) to identify SMA20/PMIS2 loci and to align deduced amino acid sequences of predicted ORFs.

Techniques: Construct